Did this ever get resolved? I have the same issue.
Audio tests enabled, flash enabled (on request), works fine on mobile (iPhone) and internet explorer (Windows 7), not tested on Firefox, never works on Chrome on either an Apple or Windows machine, unless I’m in incognito mode or logged out of Chrome with all my local data cleared.
So, must be something in my personalised Chrome settings, but do you have any leading candidates for what?
It isn’t 3rd party cookies (blocked, but I have tested it with them unblocked) and while the browser is slightly more restricted than it is in default state (sensible if you have any privacy concerns at all) it’s not so restricted that audio is affected on any other site.
More of a pain than it sounds like. I have only Chrome on my office machine (no choice in this). Incognito mode is a(n ugly) workaround but would love to have this working properly, so if you have any obvious candidates for settings to change please let me know. I’ll bet it’s the same problem affecting all of us.
Thanks a lot.